Antonio Semini (c. 1485- after 1547) was an Italian painter of the late-Renaissance, active in his native Genoa.
He was born and trained in Genoa and died in Milan. He was the pupil of Ludovico Brea, and painted in collaboration with Teramo Piaggio. Andrea painted a Nativity for S. Domenico, Savona, One of his pupils was his son Ottavio Semini.